Biologicals & Vaccine Production

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Course Code: 
BLT 3102
Course Credit Units: 
Semester 1
Year of Study: 
Year 2
Undergraduate or Graduate Level: 
Undergraduate Level
Course Discipline: 
Course Description & Objectives: 
  • Rationale for biologics and vaccines production. Basics of major biologics in bioscience; Review immunisation, reference standards and financial management. Biologics manufacturing, establishment and equipment.
  • Types of vaccines (inactivated, live vaccines); Large scale vaccine production and packaging; Viral vaccine /cell culture production
  • Genetically engineered vaccines, lympholization, QC of vaccines legal and ethical registration
  • Routes of immunisation, production of hyperimmune serum.
  • Production and evaluation of antigens and antibodies. Conjugation of immunoglobulins and monoclonal antibodies. Basic practical procedures in protein purification, conjugation, dialysis, lyophilisation and freeze-drying. Vaccine production: traditional methods versus cell production systems, Continuous cascade virus production system. Prototype vaccine production using New castle disease vaccine virus: vaccine seed strain, production of the master seed virus; laboratory facilities, production security, security of experimental animals, testing source flock, propagation facilities; Propagation of vaccine virus; Inactivated vaccines; Potency and other tests; Virus content of vaccines and Test for virus characteristics.


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