Course Description & Objectives:
- Rationale for biologics and vaccines production. Basics of major biologics in bioscience; Review immunisation, reference standards and financial management. Biologics manufacturing, establishment and equipment.
- Types of vaccines (inactivated, live vaccines); Large scale vaccine production and packaging; Viral vaccine /cell culture production
- Genetically engineered vaccines, lympholization, QC of vaccines legal and ethical registration
- Routes of immunisation, production of hyperimmune serum.
- Production and evaluation of antigens and antibodies. Conjugation of immunoglobulins and monoclonal antibodies. Basic practical procedures in protein purification, conjugation, dialysis, lyophilisation and freeze-drying. Vaccine production: traditional methods versus cell production systems, Continuous cascade virus production system. Prototype vaccine production using New castle disease vaccine virus: vaccine seed strain, production of the master seed virus; laboratory facilities, production security, security of experimental animals, testing source flock, propagation facilities; Propagation of vaccine virus; Inactivated vaccines; Potency and other tests; Virus content of vaccines and Test for virus characteristics.
